Understanding Butyrate and Why You Should Care

By Ross Pelton, RPh, PhD, CCN
Scientific Director, Essential Formulas

Butyrate, classified as a short-chain fatty acid (SCFA), is one of the most critical postbiotic metabolites produced by your intestinal bacteria. Butyrate is made when certain strains of probiotic bacteria ferment non-digestible dietary fibers in your colon.

The cells that line your colon are called colonocytes. These cells have the highest rate of turnover in the human body. Most people don’t realize that they create an entirely new lining of their colon every 5 to 7 days. It takes an enormous amount of energy for the body to be making these new cells constantly.i

Butyrate & Energy:
Butyrate is the primary energy source for the continual renewal of colonocytes. It has been estimated that approximately 10% of a human’s daily energy requirement is provided by butyrate. Hence, butyrate plays a critical role in an individual’s daily energy requirements and ensures the healthy renewal of the cells that line the colon.ii

Butyrate Creates Hypoxia (Low Oxygen) in the Colon:
Virtually every human cell in your body requires oxygen to function. However, most of your microbial cells thrive in an anaerobic (low oxygen or non-oxygen) environment. This is crucial for maintaining a healthy microbiome.

Probiotic bacteria in the colon produce the postbiotic metabolite butyrate. The cells lining the colon (colonocytes) absorb butyrate and metabolize it to produce energy. This process, called beta-oxidation, utilizes significant quantities of oxygen, which creates a low oxygen (hypoxia) condition in the colon. Over 99% of your probiotic bacteria are anaerobic, which means they thrive in a low-oxygen environment.

Think about what happens when you take antibiotics, which kills off the harmful bacteria along with the good. When your butyrate-generating bacteria are killed, colonocytes do not have access to adequate butyrate. When butyrate is in short supply, colonocyte metabolism is forced to change. As a result, the colonocytes utilize less oxygen, which causes the level of oxygen in the colon to increase. Higher oxygen content is lethal to your anaerobic probiotic bacteria, but it supports and promotes the growth of pathogens.

Inflammation & Leaky Gut:
Low levels of butyrate are associated with higher levels of inflammation and disruption of the intestinal barrier, which leads to intestinal permeability or “leaky gut.”iii Studies have shown that increasing levels of butyrate-producing gut bacteria such as Faecalibacteria reduces inflammation and helps heal intestinal permeability.

Keto Diets
Ketogenic or “keto” diets have become quite popular. The standard ketogenic diet is designed to contain very low carbohydrate (only 10%), moderate protein (20%), and high fat (70%). Keto diets are popular among bodybuilders who what to increase their muscle mass, and keto diets also result in weight loss.

While keto diets may provide some health benefits, there can be some negative consequences from dramatically lowering the intake of carbohydrates. Studies have shown people on ketogenic diets have reduced numbers of critical butyrate-producing bacteria. Lower levels of butyrate increase the risk of inflammation and damage to the gut mucosal barrier, leading to intestinal permeability.iv Thus, there may be health risks associated with long-term adherence to ketogenic diets.

How To Enhance Your Microbiome’s Butyrate Production?
The internet abounds with suggestions on how to increase your gut butyrate levels, such as consuming more butter or taking butyrate supplements. However, orally consuming butyrate isn’t very effective as the butyrate gets absorbed, which means it does not reach the colon, which is the site location where it produces its health benefits.

The only effective method of increasing butyrate levels in the colon is to let your probiotic bacteria do the work. How is that accomplished? By increasing your intake of carbohydrates in the form of fiber-rich foods such as multi-colored vegetables. Several different strains of intestinal bacteria, known for their ability to produce butyrate, reside in the colon.v By consuming a diet that contains a wide range of different kinds of fiber-rich foods, you will ensure that your strains of butyrate-producing bacteria have the fiber they need to produce optimal levels of butyrate for you.

Dr. Ohhira’s Probiotics are premier dietary supplements to support a healthy microbiome and to promote overall intestinal health. Produced through an exclusive, multi-year fermentation process, these products are made from a wide range of prebiotics from fiber-rich foods coupled with multiple synergistic strains of probiotics – known for their ability to create butyrate along with other beneficial postbiotic metabolites. Discover the Dr. Ohhira’s Advantage with a full-spectrum of prebiotics, probiotics and postbiotics.


i Arike L, et al. Protein Turnover in Epithelial Cells and Mucus along the Gastrointestinal Tract Is Coordinated by the Spatial Location and Microbiota. Cell Reports. 28 Jan 2020;30(4):1077-1087.
ii Brahe LK, et al. Is butyrate the link between diet, intestinal microbiota, and obesity-related metabolic diseases? Obes Rev. 2013 Dec;14(12):950-959.
iii Knudsen K, et al. Impact of Diet-Modulated Butyrate Production on Intestinal Barrier Function and Inflammation. Nutrients. 2018 Oct 13;10(10):1499.
iv Landefeld M, et al. The ketogenic diet influences taxonomic and functional composition of the gut microbiota in children with severe epilepsy. NPJ Biofilms Microbiomes. 2019;5:5.
v Zhao J, et al. Dietary Fiber Increases Butyrate-Producing Bacteria and Improves the Growth Performance of Weaned Piglets. J Agric Food Chem. 2018 Aug 1;66(30):7995-8004.